Produção de enzimas extracelulares por Crinipellis perniciosa

Production and Germination of Conidia of Trichoderma stromaticum, a Mycoparasite of Crinipellis perniciosa on Cacao.

ABSTRACT Growth characteristics of the fungus Trichoderma stromaticum, a mycoparasite on the mycelium and fruiting bodies of Crinipellis perniciosa, the causal agent of witches'-broom disease of cacao, were evaluated under controlled environmental conditions. The ability of T. stromaticum to produce conidia and germinate on dry brooms was evaluated at three constant temperatures (20, 25, and 30...

PCR Amplification and Sequence Analyses of Reverse Transcriptase- like Genes in Crinipellis perniciosa Isolates

Reverse transcriptase (RT) sequence analysis is an important technique used to detect the presence of transposable elements in a genome. Putative RT sequences were analyzed in the genome of the pathogenic fungus C. perniciosa, the causal agent of witches’ broom disease of cocoa. A 394 bp fragment was amplified from genomic DNA of different isolates of C. perniciosa belonging to C-, L-, and S-bi...

Rapid and efficient protocol for DNA extraction and molecular identification of the basidiomycete Crinipellis perniciosa.

DNA isolation from some fungal organisms is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. Beginning with a yeast Saccharomyces cerevisiae genomic DNA isolation method, we developed a 30-min DNA isolation protocol for filamentous fungi by combining cell wall digestion with cell disruption by glass beads. High-quality DNA was isolated with good yie...

Análisis del algoritmo de optimización por enjambre de partículas por medio de una aplicación gráfica 3D

Particle swarm optimization method has become popular in recent years, because of its efficiency and low computational cost. In this document, a brief analysis of the standard algorithm is performed by a 3D application. The source code of the application is free software and you can download at GitHub.

Estudo Bibliométrico da Produção Científica de Gerenciamento de Riscos entre os Anos de 2000 e 2012